Breaking the Bottleneck in the Protein Biomarker Pipeline
نویسندگان
چکیده
منابع مشابه
Breaking the bottleneck in the protein biomarker pipeline.
The process for discovery and development of biomarkers of solid tumors presents exceptional challenges. The major technical obstacle is the disconnection between the site of their generation (tissue, proximal fluid) and the source of detection (body fluids, predominantly serum and plasma). This single factor limits, in practical terms, the potential pool of biomarkers to those that are secrete...
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BACKGROUND Although robust discovery-phase platforms have resulted in the generation of large numbers of candidate cancer biomarkers, a comparable system for subsequent quantitative assessment and verification of all candidates is lacking. Established immunoassays and available antibodies permit analysis of small subsets of candidates; however, the lack of commercially available reagents, coupl...
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Proteomics has evolved over the past 10 years from a method providing mainly qualitative information— protein identification and modification site determination—to a more quantitative technique. Until quite recently, quantitative proteomics was focused on relative quantification for biomarker discovery. Biomarker discovery, by definition, must take a “shotgun” approach, which usually involves a...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2012
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2011.175034